On the application of affinity chromatography to turnover studies on the lactate dehydrogenase isoenzymes

Don M. and Masters C.J. (1975) On the application of affinity chromatography to turnover studies on the lactate dehydrogenase isoenzymes. BBA - Enzymology, 384 1: 25-36. doi:10.1016/0005-2744(75)90092-3


Author Don M.
Masters C.J.
Title On the application of affinity chromatography to turnover studies on the lactate dehydrogenase isoenzymes
Journal name BBA - Enzymology
ISSN 0005-2744
Publication date 1975-03-28
Sub-type Article (original research)
DOI 10.1016/0005-2744(75)90092-3
Volume 384
Issue 1
Start page 25
End page 36
Total pages 12
Subject 2700 Medicine
Abstract 1. 1.The suitability of a combined application of the techniques of affinity chromatography, double labelling and gel electrophoresis in the determination of the turnover characteristics of the lactate dehydrogenases isoenzymes (l-lactate:NAD+ oxidoreductase EC 1.1.1.27) in rat tissues has been studied. 2. 2.Affinity chromatography was established as affording the advantages of rapidity, high yield and purity to such studies, and the double-labelling procedure was modified to encompass the differential decay kinetics in the separate rat tissues. In addition, a convenient method for the resolution and separate collection of radioactively labelled isoenzymes has been described. 3. 3.Using this methodology, comparative turnover values for the isoenzymes of lactate dehydrogenase and for total soluble protein have been determined. 4. 4.The comparability of these results with other methodologies, and the advantages of this approach in facilitating broad comparative studies on turnover are discussed.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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