Improved molecular weight analysis of streptococcal hyaluronic acid by size exclusion chromatography

Armstrong D.C. and Johns M.R. (1995) Improved molecular weight analysis of streptococcal hyaluronic acid by size exclusion chromatography. Biotechnology Techniques, 9 7: 491-496. doi:10.1007/BF00159564


Author Armstrong D.C.
Johns M.R.
Title Improved molecular weight analysis of streptococcal hyaluronic acid by size exclusion chromatography
Journal name Biotechnology Techniques   Check publisher's open access policy
ISSN 0951-208X
Publication date 1995-01-01
Sub-type Article (original research)
DOI 10.1007/BF00159564
Volume 9
Issue 7
Start page 491
End page 496
Total pages 6
Publisher Kluwer Academic Publishers
Subject 2402 Applied Microbiology and Biotechnology
1303 Specialist Studies in Education
Abstract Four size exclusion chromatography (SEC) calibration techniques were tested for use in the molecular weight characterisation of Streptococcal Hyaluronic Acid (HA). An exponential equation, evaluated using the Hamielec method, was superior to the customary peak position method. It provided the most accurate estimates of the weight average molecular weight, Mw. The calibration was valid for HA in the range 800 - 2500 kDa, and permitted the calculation of both polydispersity and molecular weight distributions for HA from Streptococcal fermentations. This exponential calibration approach should have application in the characterisation of other large biopolymers, particularly where pore size of available SEC media is limiting.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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