Digestion of 14C-labelled condensed tannins from Desmodium intortum in sheep and goats

Perez-Maldonado, R. A. and Norton, B. W. (1996) Digestion of 14C-labelled condensed tannins from Desmodium intortum in sheep and goats. British Journal of Nutrition, 76 4: 501-513. doi:10.1079/BJN19960059

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Author Perez-Maldonado, R. A.
Norton, B. W.
Title Digestion of 14C-labelled condensed tannins from Desmodium intortum in sheep and goats
Formatted title
Digestion of 14C-labelled condensed tannins from Desmodium intortum in sheep and goats
Journal name British Journal of Nutrition   Check publisher's open access policy
ISSN 0007-1145
Publication date 1996-10-01
Sub-type Article (original research)
DOI 10.1079/BJN19960059
Open Access Status File (Publisher version)
Volume 76
Issue 4
Start page 501
End page 513
Total pages 13
Place of publication Cambridge, United Kingdom
Publisher Cambridge University Press
Language eng
Formatted abstract
An experiment was conducted to investigate the metabolism of condensed tannin (CT) in sheep and goats offered a mixture of Digitaria decumbens (700 g/kg) and Desmodium intortum (300 g/kg) hay. Radioactive 14CO2 was used to label CT in young growing desmodium plants, [14C]CT was extracted, purified and infused intraruminally, and the metabolism of [14C]CT was followed in the rumen and lower digestive tract of both species. Digestion of DM, organic matter (OM), cell-wall constituents (CWC), N and the efficiency of rumen microbial synthesis were determined using a continuous intraruminal infusion of 51Cr EDTA, YbCl3 and Na235SO4. The measurements taken for sheep and goats respectively were: intake, 21 and 30 g/kg0.9 per d; digestibilities (g/g) of DM, 0.566 and 0.505; OM 0.578 and 0.508; neutral-detergent fibre, 0.584 and 0.532; and acid-detergent fibre, 0.535 and 0.435. None of these measurements was significantly different (P > 0.05) between animal species. There was an apparent net gain in Lignin across the rumen and whole intestinal tract for both animal species (19 and 29% for sheep and goats respectively). There were no significant differences between sheep and goats (P > 0.05) detected for any measurements of N excretion and utilization. The overall efficiency of N digestion and utilization was also similar between species. The routes of CT metabolism mere compared for both colorimetric estimates (butanol-HCl) of dietary CT (DCT) and the specific radioactivity of [14C]CT in digesta (abomasum) and excreta (urine and faeces) of both sheep and goats. [14C]CT showed total losses of 57 and 56% in sheep and goats respectively whilst losses of DCT of 71 and 70% were detected with butanol-HCl in sheep and goats respectively. The apparent losses of DCT across the rumen of sheep and goats were 12 and 9% whilst higher losses (49 and 42% for sheep and goats respectively) were observed for [14C]CT. Losses of DCT in the lower intestinal tract accounted for 69 and 71% of the total CT leaving the abomasum. By comparison, only 40 and 35% of [14]CT was lost during intestinal passage in sheep and goats respectively. It was concluded that the infused free [14C]CT interacted with DCT and entered the protein and fibre-bound DCT pools. The loss of DCT during passage through the intestines was considered to be a consequence of either absorption of free CT or the degradation products of CT. It was assumed that free CT arose in the lower gastrointestinal tract from protein-CT and fibre-CT dissociation to be digested and/or absorbed. The higher recoveries of [14C]CT in faeces (32 and 35%) compared with DCT (27 and 26%) for sheep and goats respectively) were associated with the excretion of [14C] degradation products or conjugates which were not reactive to butanol-HCl. It was concluded that both methods (butanol-HCl and labelling CT with 14C) detected a substantial disappearance of CT (free, protein, and fibre-bound) during metabolism in the gastrointestinal tract in sheep and goats.
Keyword Condensed tannin
Tannin metabolism
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
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