The influence of dissolved oxygen concentration on phosphofructokinase and the glucose metabolism of Escherichia coli K-12

Reichelt J.L. and Doelle H.W. (1971) The influence of dissolved oxygen concentration on phosphofructokinase and the glucose metabolism of Escherichia coli K-12. Antonie van Leeuwenhoek, 37 1: 497-506. doi:10.1007/BF02218520


Author Reichelt J.L.
Doelle H.W.
Title The influence of dissolved oxygen concentration on phosphofructokinase and the glucose metabolism of Escherichia coli K-12
Journal name Antonie van Leeuwenhoek   Check publisher's open access policy
ISSN 0003-6072
Publication date 1971-01-01
Sub-type Article (original research)
DOI 10.1007/BF02218520
Volume 37
Issue 1
Start page 497
End page 506
Total pages 10
Publisher Kluwer Academic Publishers
Subject 2404 Microbiology
1312 Molecular Biology
Abstract The glucose metabolism and the response of phosphofructokinase activity to oxygen were investigated using glucose-limited chemostat cultures of E. coli K-12. With a dilution rate of 0.2 hr-1 and a glucose input concentration of 0.83 g/litre, 10 steady states were obtained ranging from 320 to 0 mm HgO2. Dissolved oxygen reached zero level at a pO2 of 25.8 mm Hg. The specific phosphofructokinase activity was constant above 28 mm Hg O2 and increased linearly at lower pO2 levels until it reached highest activity at 0 mm Hg O2. Cell dry weight also started to decrease linearly from 28 to 5.9 mm Hg O2, and fell sharply thereafter. Acid production rate did not start before pO2 reached 25.6 mm Hg, increased progressively with an additional sharp increase below 5.9 mm Hg O2. The main endproducts formed were acetic acid and ethanol with lactic acid appearing below 5.9 mm Hg O2. The results suggest an effect of oxygen on phosphofructokinase synthesis rather than an ATP inhibition of the enzyme.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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