A one-step process for the production of single-cell protein and amyloglucosidase

Sukara E. and Doelle H.W. (1989) A one-step process for the production of single-cell protein and amyloglucosidase. Applied Microbiology and Biotechnology, 30 2: 135-140. doi:10.1007/BF00264000


Author Sukara E.
Doelle H.W.
Title A one-step process for the production of single-cell protein and amyloglucosidase
Journal name Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0175-7598
Publication date 1989-01-01
Sub-type Article (original research)
DOI 10.1007/BF00264000
Volume 30
Issue 2
Start page 135
End page 140
Total pages 6
Publisher Springer-Verlag
Subject 2726 Microbiology (medical)
2404 Microbiology
1502 Banking, Finance and Investment
1305 Biotechnology
Abstract A new Rhizopus species was isolated from traditional Indonesian food, tempeh. The newly isolated species was similar in its morphological characteristics to Rhizopus oligosporus UQM 145F, but grew faster on potato-dextrose agar as well as in submerged culture. The new isolate was found to convert ground cassava tuber directly into single cell protein without pretreatment due to its high amyloglucosidase formation. From 100 g ground tuber, a dry biomass of 33.75 g containing 26.48% true protein together with 60 ml of highly active amyloglucosidase (282 units) was obtained in 12 h. The amyloglucosidase was recovered by ultrafiltration, releasing 26.226 millimol glucose/l/min from soluble starch. The crude enzyme exhibited a pH optimum between 4.6 and 5.0, a temperature optimum between 55 and 60° C and an apparent Km of 3.125 g/l. High substrate concentrations and ammonium sulphate are inhibitory to the enzyme.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 05 Jul 2016, 12:02:34 EST by System User