Effect of phytolectins on isolated protoplasts from plants

Chin J.C. and Scott K.J. (1979) Effect of phytolectins on isolated protoplasts from plants. Annals of Botany, 43 1: 33-44.

Author Chin J.C.
Scott K.J.
Title Effect of phytolectins on isolated protoplasts from plants
Journal name Annals of Botany   Check publisher's open access policy
ISSN 0305-7364
Publication date 1979-01-01
Sub-type Article (original research)
Volume 43
Issue 1
Start page 33
End page 44
Total pages 12
Subject 1804 Statistics, Probability and Uncertainty
2604 Applied Mathematics
3207 Social Psychology
3203 Clinical Psychology
2743 Reproductive Medicine
1110 Nursing
1108 Medical Microbiology
Abstract The effects of three phytolectins on isolated plant protoplasts have been examined. It was found that concanavalin A, phytohemagglutinin and wheat germ agglutinin were all capable of agglutinating protoplasts isolated from a variety of plant species. The incorporation of 3H-uridine, 3H-thymidine and 14C-leucine into RNA, DNA and protein respectively was also stimulated by the three lectins in isolated protoplasts. Treatment with α-methyl-D-mannoside abolished both the agglutinating and incorporation enhancing effects of concanavalin A (Con A) on barley protoplasts. Protoplasts with a regenerated cell wall also failed to respond to lectin treatment. These results suggest that lectin-binding to the protoplast membrane surface is required before either agglutination or metabolic stimulation will result.
Keyword Agglutination
Isolated protoplasts
Lectins
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 05 Jul 2016, 11:45:29 EST by System User