Concentration-dependent migration in gel chromatography of reversibly polymerizing solutes: the osmotic behavior of polyacrylamide gels

Tellam R. and Winzor D.J. (1980) Concentration-dependent migration in gel chromatography of reversibly polymerizing solutes: the osmotic behavior of polyacrylamide gels. Biophysical Chemistry, 12 3-4: 299-306. doi:10.1016/0301-4622(80)80007-X


Author Tellam R.
Winzor D.J.
Title Concentration-dependent migration in gel chromatography of reversibly polymerizing solutes: the osmotic behavior of polyacrylamide gels
Journal name Biophysical Chemistry   Check publisher's open access policy
ISSN 0301-4622
Publication date 1980-01-01
Sub-type Article (original research)
DOI 10.1016/0301-4622(80)80007-X
Volume 12
Issue 3-4
Start page 299
End page 306
Total pages 8
Subject 1303 Specialist Studies in Education
1304 Biophysics
1606 Political Science
Abstract The osmotic behavior of gels derived from polyacrylamide has been measured in order to establish the validity of assuming linear concentration dependence of bead shrinkage upon osmotic pressure in quantitative studies of reversibly associating solutes by gel chromatography on Bio-Gel. The concentration dependence of elution volume predicted with due allowance for this osmotic shrinkage yields theoretical curves that provide good descriptions of experimental results obtained in chromatography of α-chymotrypsin on Bio-Gel P-30 equilibrated with acetate-chloride buffer, pH 3-86, 10.20; and also of bacterial α-amylase on Bio-Gel P-150 equilibrated with 0.10 M NaCl-0.015 M calcium acetate-0.010 M EDTA, pH 7.0. For the former system the osmotic effect has negligible consequences on the quantitative interpretation of the results. With the α-amylase system, however, consideration of the osmotic effect is necessary to obtain even a qualitative indication of the existence of the monomer-dimer equilibrium.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 05 Jul 2016, 11:43:23 EST by System User