Advantages in the use of aldehyde fuchsin with van gieson's picro-fuchsin counterstaining for differentiating bone and cartilage

Addison P.C. (1973) Advantages in the use of aldehyde fuchsin with van gieson's picro-fuchsin counterstaining for differentiating bone and cartilage. Biotechnic and Histochemistry, 48 2: 59-62. doi:10.3109/10520297309116582


Author Addison P.C.
Title Advantages in the use of aldehyde fuchsin with van gieson's picro-fuchsin counterstaining for differentiating bone and cartilage
Journal name Biotechnic and Histochemistry   Check publisher's open access policy
ISSN 1052-0295
Publication date 1973-01-01
Sub-type Article (original research)
DOI 10.3109/10520297309116582
Volume 48
Issue 2
Start page 59
End page 62
Total pages 4
Publisher Informa Healthcare
Subject 2722 Histology
3607 Medical Laboratory Technology
Abstract Specimens of bone were fixed in 10% neutral phosphate-buffered formalin or in Bouin's fluid and decalcified in 10% formic acid buffered with 10% sodium citrate. Materials were embedded in paraffin and 4-5 μ sections attached to slides were oxidized with 0.5% KMnO4, decolorized in 1% oxalic acid, stained with aldehyde fuchsin, and counter-stained with Van Gieson's picro-fuchsin. Sections were dehydrated, cleared and mounted in a synthetic resin. Microscopically, the differentiation between bone and cartilage was seen as red and purple respectively, with connective tissue red; muscle and erythrocytes, yellow; and elastic fibres purple. The areas occupied by bone, cartilage and erythrocytes could be compared, and also the depth to which cartilage extended into the ossified sites. The advantages of this staining combination are: good contrasts in colour, ease of applying the stain, and virtual self-differentiation of the staining solutions.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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