Bactericidal action of ascorbic acid on pseudomonas aeruginosa: Alteration of cell surface as a possible mechanism

Rawal B.D. (1978) Bactericidal action of ascorbic acid on pseudomonas aeruginosa: Alteration of cell surface as a possible mechanism. Chemotherapy, 24 3: 166-171. doi:10.1159/000237777


Author Rawal B.D.
Title Bactericidal action of ascorbic acid on pseudomonas aeruginosa: Alteration of cell surface as a possible mechanism
Journal name Chemotherapy   Check publisher's open access policy
ISSN 1421-9794
Publication date 1978-01-01
Sub-type Article (original research)
DOI 10.1159/000237777
Volume 24
Issue 3
Start page 166
End page 171
Total pages 6
Subject 2725 Infectious Diseases
2730 Oncology
2736 Pharmacology (medical)
3002 Drug Discovery
3004 Pharmacology
Abstract Neutralised ascorbic acid is found to exert a strong bactericidal action on Pseudomonas aeruginosa suspended in isotonic phosphate buffer at pH 7.1. Both the bactericidal and bacteriostatic action of ascorbic acid are antagonised by magnesium ions. In the absence of complex formation between magnesium and ascorbic acid it is concluded that ascorbic acid acts by competing with the magnesium binding sites in the cell wall, cell membrane or ribosomes. Using the chequer-board titration method the synergistic action of ascorbic acid and erythromycin is determined; such a potentiation of erythromycin is also adversely affected by magnesium ions. P. aeruginosa cells, washed and suspended in isotonic phosphate buffer containing ascorbic acid, became increasingly susceptible to the action of polymyxin, erythromycin, chloramphenicol, neomycin and tetracycline. It is suggested that ascorbic acid alters the cell surface to render it increasingly permeable to these antibiotics.
Keyword Antimicrobial agent
Ascorbic acid
Chloramphenicol
Erythromycin
Neomycin
Polymyxin
Pseudomonas
Synergy
Tctracycline
Vitamin C
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 05 Jul 2016, 10:46:39 EST by System User