Quantitation of Morphine, Morphine-3-Glucuronide, and Morphine-6-Glucuronide in Plasma and Cerebrospinal-Fluid Using Solid-Phase Extraction and High-Performance Liquid-Chromatography with Electrochemical Detection

Wright, Awe, Watt, JA, Kennedy, M, Cramond, T and Smith, MT (1994) Quantitation of Morphine, Morphine-3-Glucuronide, and Morphine-6-Glucuronide in Plasma and Cerebrospinal-Fluid Using Solid-Phase Extraction and High-Performance Liquid-Chromatography with Electrochemical Detection. Therapeutic Drug Monitoring, 16 2: 200-208. doi:10.1097/00007691-199404000-00016


Author Wright, Awe
Watt, JA
Kennedy, M
Cramond, T
Smith, MT
Title Quantitation of Morphine, Morphine-3-Glucuronide, and Morphine-6-Glucuronide in Plasma and Cerebrospinal-Fluid Using Solid-Phase Extraction and High-Performance Liquid-Chromatography with Electrochemical Detection
Journal name Therapeutic Drug Monitoring   Check publisher's open access policy
ISSN 0163-4356
Publication date 1994-04-01
Year available 1994
Sub-type Article (original research)
DOI 10.1097/00007691-199404000-00016
Open Access Status Not yet assessed
Volume 16
Issue 2
Start page 200
End page 208
Total pages 9
Place of publication PHILADELPHIA
Publisher LIPPINCOTT-RAVEN PUBL
Language eng
Subject 3004 Pharmacology
2736 Pharmacology (medical)
Abstract An original, sensitive, and specific high-performance liquid chromatographic (HPLC) assay was developed for the quantitation of morphine and its two major metabolites, morphine-3-glucuronide (M3G) and morphine-6-glucuronide (M6G), in human plasma and cerebrospinal fluid (CSF) and in rat plasma, using hydromorphone as the internal standard. Solid-phase extraction was used to separate morphine and its glucuronide metabolites from plasma constituents. Extraction efficiencies of morphine, M3G, and M6G from human plasma samples (0.5 ml) were 84, 87, and 88%, respectively. Extraction efficiencies of morphine, M3G, and M6G did not differ significantly (p > 0.05) between human plasma and CSF or rat plasma. Morphine, M3G, M6G, and hydromorphone were separated on a 10 mu C-8 Resolve radially compressed cartridge using a mobile phase comprising methanol:acetonitrile:phosphate buffer, (0.0125M pH 7.5; 10: 10:80), in which 11 mg/L of cetyltrimethylammonium bromide (cetrimide) was dissolved. Quantitation was achieved using a single electrochemical detector at ambient temperature (23-degrees-C). Standard curves were linear over the ranges 0.020-2.190, 0.027-2.709, and 0.027-0.542 muM for morphine, M3G, and M6G, respectively. Lower limits of detection for morphine, M3G, and M6G in human plasma and CSF samples (0.5 ml) were 0.020, 0.027, and 0.027 muM, respectively. Corresponding lower limits of detection in rat plasma (0.1 ml) were 0.102, 0.135, and 0.135 muM, respectively. Intraassay precision for low and high concentrations of morphine, M3G, and M6G were <23 and <8% respectively. Similarly, interassay accuracy for low and medium concentrations of morphine, M3G, and M6G were <17% and were <9% for high concentrations.
Keyword Morphine
Morphine-3-Glucuronide
Morphine-6-Glucuronide
High-Performance Liquid Chromatography
Electrochemical Detection
Solid Phase Extraction
Optical-Detection
Cancer-Patients
Metabolites
Rats
Pharmacokinetics
Multiwavelength
Hydromorphone
Codeine
Urine
Csf
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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