A sensitive liquid chromatographic assay for plasma aspirin and salicylate concentrations after low doses of aspirin

Brandon R.A., Eadie M.J. and Smith M.T. (1985) A sensitive liquid chromatographic assay for plasma aspirin and salicylate concentrations after low doses of aspirin. Therapeutic Drug Monitoring, 7 2: 216-221. doi:10.1097/00007691-198506000-00014


Author Brandon R.A.
Eadie M.J.
Smith M.T.
Title A sensitive liquid chromatographic assay for plasma aspirin and salicylate concentrations after low doses of aspirin
Journal name Therapeutic Drug Monitoring   Check publisher's open access policy
ISSN 1536-3694
Publication date 1985-01-01
Year available 1985
Sub-type Article (original research)
DOI 10.1097/00007691-198506000-00014
Open Access Status Not yet assessed
Volume 7
Issue 2
Start page 216
End page 221
Total pages 6
Place of publication PHILADELPHIA
Publisher LIPPINCOTT-RAVEN PUBL
Language eng
Subject 2736 Pharmacology (medical)
3004 Pharmacology
1300 Biochemistry, Genetics and Molecular Biology
1303 Specialist Studies in Education
2307 Health, Toxicology and Mutagenesis
2739 Public Health, Environmental and Occupational Health
3005 Toxicology
Abstract An original, highly sensitive and specific high performance liquid chromatographic method has been developed for the measurement of aspirin and salicylate in plasma. Minimum concentrations of 10 xg/L (aspirin) and 0.5 mg/L (salicylate) can be measured using 1 ml of plasma. After collection, plasma is first treated with physostigmine sulfate to inhibit enzymatic hydrolysis of aspirin to salicylate. Maximal recovery is achieved using an acid extraction into anhydrous diethyl ether with a subsequent drying-down step in an iced water bath. Aspirin and salicylate are separated by elution with a mixture of methanol, 1-butanol, orthophosphoric acid, and water on a reversed-phase octadecyl silane column at 47°C and detected at 234 nm by ultraviolet absorption. Quantitation is achieved using the peak height ratio of aspirin and salicylate to internal standard (m-anisic acid). The assay has been used for the study of simultaneous aspirin and salicylate pharmacokinetics after a single oral dose of 100 mg soluble glycinated aspirin for platelet antiaggregatory therapy in six subjects, one of whom was also studied after receiving a 600 mg dose.
Keyword Aspirin
Liquid chromatography
Pharmacokinetics
Salicylate
Thromboembolism
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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