In vitro propagation of cassava (Manihot esculenta Crantz)

Smith M.K., Biggs B.J. and Scott K.J. (1986) In vitro propagation of cassava (Manihot esculenta Crantz). Plant Cell, Tissue and Organ Culture, 6 3: 221-228. doi:10.1007/BF00040007


Author Smith M.K.
Biggs B.J.
Scott K.J.
Title In vitro propagation of cassava (Manihot esculenta Crantz)
Journal name Plant Cell, Tissue and Organ Culture   Check publisher's open access policy
ISSN 0167-6857
Publication date 1986-01-01
Sub-type Article (original research)
DOI 10.1007/BF00040007
Open Access Status Not yet assessed
Volume 6
Issue 3
Start page 221
End page 228
Total pages 8
Publisher Martinus Nijhoff, The Hague/Kluwer Academic Publishers
Subject 1305 Biotechnology
1110 Nursing
Abstract A method is presented for the rapid in vitro propagation of cassava (Manihot esculenta Crantz). Nodal explants were induced to grow as multiple-shoot cultures on a medium containing 1.0 μM 6-benzylamino purine (BAP), supplemented with 0.25 μM α-naphthaleneacetic acid (NAA). Nodes were removed from the shoots after three weeks of growth and subcultured on fresh culture medium. An average of 7.0 nodes were produced from each explanted node after three weeks in culture. Nodal explants were transferred to a medium containing 2.5 μM indole-3-butyric acid (IBA) to improve root initiation on the developing plantlets. Plant establishment was possible upon transfer to soil. In vitro propagation offers enhanced rates of multiplication over more conventional methods of propagation. In addition, in vitro propagation facilitates the storage and international exchange of cassava germplasm.
Keyword cassava
in vitro propagation
Manihot esculenta
propagation
tissue culture
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 28 Jun 2016, 18:12:26 EST by System User