Use of DNA from dry leaves for PCR and RAPD analysis

Thomson D. and Henry R. (1993) Use of DNA from dry leaves for PCR and RAPD analysis. Plant Molecular Biology Reporter, 11 3: 202-206. doi:10.1007/BF02669845


Author Thomson D.
Henry R.
Title Use of DNA from dry leaves for PCR and RAPD analysis
Journal name Plant Molecular Biology Reporter   Check publisher's open access policy
ISSN 0735-9640
Publication date 1993-01-01
Sub-type Article (original research)
DOI 10.1007/BF02669845
Volume 11
Issue 3
Start page 202
End page 206
Total pages 5
Publisher Kluwer Academic Publishers
Subject 1303 Specialist Studies in Education
1300 Biochemistry, Genetics and Molecular Biology
1110 Nursing
Abstract Fresh or frozen tissue is usually used as a source of DNA for PCR and RAPD analysis. We have found that leaves can be allowed to dry at room temperature before extraction of DNA. Heating the leaves or microwave drying resulted in poor recovery of DNA. Storage of fresh leaves in paper envelopes in the laboratory was the most successful approach. This allowed the tissue to dry out over a period of several days and DNA could be extracted at any time, providing a convenient method for the collection and analysis of field material. DNA from leaves stored for four months at room temperature was suitable for PCR analysis.
Keyword DNA extraction
PCR
RAPD
sample preparation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 24 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 28 Jun 2016, 17:47:39 EST by System User