Evidence for lipid involvement in the high-affinity interaction between cimetidine and rat liver cytochrome P-450

Ioannoni B., Reilly P.E.B. and Winzor D.J. (1985) Evidence for lipid involvement in the high-affinity interaction between cimetidine and rat liver cytochrome P-450. Archives of Biochemistry and Biophysics, 241 1: 81-87. doi:10.1016/0003-9861(85)90364-9


Author Ioannoni B.
Reilly P.E.B.
Winzor D.J.
Title Evidence for lipid involvement in the high-affinity interaction between cimetidine and rat liver cytochrome P-450
Journal name Archives of Biochemistry and Biophysics   Check publisher's open access policy
ISSN 1096-0384
Publication date 1985-08-15
Sub-type Article (original research)
DOI 10.1016/0003-9861(85)90364-9
Volume 241
Issue 1
Start page 81
End page 87
Total pages 7
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract The spectral interaction between cimetidine and a cytochrome P-450 fraction isolated from liver of untreated rats has been shown to be markedly affected by dilauroylphosphatidylcholine. In the absence of the lipid the pigment preparation yielded a binding curve characteristic of a single isoenzyme species with low affinity for the drug, whereas its inclusion led to the observation of a much stronger interaction with a dissociation constant close to that obtained for the high-affinity component(s) of the parent microsomes; material with lower affinity was also observed. Gel chromatography and partition equilibrium studies yielded results which precluded interpretation of this finding either in terms of incomplete incorporation of the pigment into the phospholipid or of disproportionate solvation of cimetidine into the lipid phase of the reconstituted phospholipid complex. In contrast, phospholipid caused only a minor change in the strength of cimetidine binding by the predominant liver cytochrome P-450 from phenobarbitone-pretreated rats. Pronounced lipid sensitivity of cimetidine-binding affinity is thus not a general feature of the microsomal cytochrome P-450 system but rather a specific characteristic of individual isoenzyme species.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 28 Jun 2016, 17:00:13 EST by System User