Studies of lectin-carbohydrate interactions by quantitative affinity chromatography: Systems with galactose and ovalbumin as saccharidic ligand

Hogg P.J. and Winzor D.J. (1987) Studies of lectin-carbohydrate interactions by quantitative affinity chromatography: Systems with galactose and ovalbumin as saccharidic ligand. Analytical Biochemistry, 163 2: 331-338. doi:10.1016/0003-2697(87)90232-6


Author Hogg P.J.
Winzor D.J.
Title Studies of lectin-carbohydrate interactions by quantitative affinity chromatography: Systems with galactose and ovalbumin as saccharidic ligand
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
Publication date 1987-01-01
Sub-type Article (original research)
DOI 10.1016/0003-2697(87)90232-6
Volume 163
Issue 2
Start page 331
End page 338
Total pages 8
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract The potential of affinity chromatography for characterizing lectin-carbohydrate interactions is investigated. First, the effect of galactose on the chromatographic behavior of Ricinus communis phytohemagglutinin on Sepharose 4B is used to establish that quantitative affinity chromatography on polysaccharide matrices affords an unequivocal means of characterizing the interactions of lectins with monosaccharides in solution. Second, a method of characterizing lectin-glycoprotein interactions by affinity chromatography is illustrated in an experimental study with Sephadex G-50 as affinity matrix for examination of the interaction between concanavalin A and ovalbumin. Third, although no general solution to the problem of ligand multivalency in quantitative affinity chromatography has been found, an experimental protocol has been devised for the situation in which the partitioning solute (lectin) is univalent.
Keyword lectin-glycoprotein interactions
ligand binding
ovalbumin-concanavalin A interaction
phytohemagglutinin-carbohydrate interactions
quantitative affinity chromatography
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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