Glucose transport and hypoxia-reoxygenation injury in the perfused rat liver

Le Couteur D.G., Rivory L.P. and Pond S.M. (1994) Glucose transport and hypoxia-reoxygenation injury in the perfused rat liver. Journal of Gastroenterology and Hepatology (Australia), 9 4: 385-390. doi:10.1111/j.1440-1746.1994.tb01260.x

Author Le Couteur D.G.
Rivory L.P.
Pond S.M.
Title Glucose transport and hypoxia-reoxygenation injury in the perfused rat liver
Journal name Journal of Gastroenterology and Hepatology (Australia)   Check publisher's open access policy
ISSN 0815-9319
Publication date 1994-01-01
Sub-type Article (original research)
DOI 10.1111/j.1440-1746.1994.tb01260.x
Open Access Status Not yet assessed
Volume 9
Issue 4
Start page 385
End page 390
Total pages 6
Publisher Blackwell Publishing
Language eng
Subject 2715 Gastroenterology
2721 Hepatology
Abstract Abstract During liver transplantation, oxidative stress occurs during hypoxia and reoxygenation of the donor organ. Chemical oxidative stress impairs cell membrane transport. Therefore, in this study the influence of hypoxia and reoxygenation on hepatocellular membrane transport was investigated. Specifically, glucose transport was studied in the perfused rat liver using the multiple indicator‐dilution technique. First, it was observed that in normal rat livers, glucose transport was rapid but saturable (K48 ± 10 mmol/L and V9.4 ± 0.9 μmol/s per g of liver). To simulate hypoxia and reoxygenation, livers were perfused for 30 min with nitrogen‐saturated buffer and then with oxygen‐saturated buffer for 20 min. The livers from fed rats were protected from hypoxia‐reoxygenation injury whereas those from fasted rats were highly susceptible to injury as determined by lactate dehydrogenase release. After reoxygenation, the rate of glucose influx decreased significantly by ∼50% in the fasted livers (P < 0.001) but was unaffected in the fed livers. This impairment of the hepatocellular transport of glucose, which could be secondary to oxidative injury to the hepatocyte membrane, has implications for the function of donor livers that have sustained hypoxia‐reoxygenation (‘preservation') injury during transplantation. Copyright
Keyword glucose transport
hypoxia-reoxygenation injury
multiple indicator- dilution
perfused rat liver
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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