Differentiation between pathogenic and non-pathogenic Yersinia enterocolitica strains by colony hybridization with a PCR-mediated digoxigenin-dUTP-labelled probe

Ibrahim A., Liesack W. and Stackebrandt E. (1992) Differentiation between pathogenic and non-pathogenic Yersinia enterocolitica strains by colony hybridization with a PCR-mediated digoxigenin-dUTP-labelled probe. Molecular and Cellular Probes, 6 2: 163-171. doi:10.1016/0890-8508(92)90061-2


Author Ibrahim A.
Liesack W.
Stackebrandt E.
Title Differentiation between pathogenic and non-pathogenic Yersinia enterocolitica strains by colony hybridization with a PCR-mediated digoxigenin-dUTP-labelled probe
Journal name Molecular and Cellular Probes   Check publisher's open access policy
ISSN 0890-8508
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1016/0890-8508(92)90061-2
Open Access Status Not yet assessed
Volume 6
Issue 2
Start page 163
End page 171
Total pages 9
Subject 1307 Cell Biology
1312 Molecular Biology
Abstract The Polymerase Chain Reaction (PCR) method was used to generate a vector-free digoxigenin-dUTP labelled probe that targets the Yersinia enterocolitica gene encoding the heat stable enterotoxin (yst). The probe was used in DNA-DNA colony hybridization to screen 113 strains of Y. enterocolitica and related species for the presence of the enterotoxin gene. In Y. enterocolitica, the probe clearly discriminated between pathogenic and non-pathogenic strains even those belonging to the same serotype. Of the other Yersinia species, only three strains of Y. kristensenii possessed DNA sequences homologous to the yst gene. The probe was further checked for its specificity in artificially inoculated fecal samples and could easily detect the target sequence of the yst gene. The digoxigenin-labelled probe proved to be a reliable epidemiological tool to discriminate between pathogenic and nonpathogenic strains in pure and mixed culture, thus offering the advantage of using a non-radioactive detection system in clinical laboratories with the possibility of reusing the same hybridization solution several times and obtaining results within a relatively short time.
Keyword DNA-DNA colony hybridization
non-radioactive DNA probe
Yersinia enterocolitica
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 28 Jun 2016, 15:41:44 EST by System User