Species differentiation in the didymozoidae (Digenea): Restriction fragment length differences in internal transcribed spacer and 5.8S ribosomal DNA

Anderson G.R. and Barker S.C. (1993) Species differentiation in the didymozoidae (Digenea): Restriction fragment length differences in internal transcribed spacer and 5.8S ribosomal DNA. International Journal for Parasitology, 23 1: 133-136. doi:10.1016/0020-7519(93)90106-9


Author Anderson G.R.
Barker S.C.
Title Species differentiation in the didymozoidae (Digenea): Restriction fragment length differences in internal transcribed spacer and 5.8S ribosomal DNA
Journal name International Journal for Parasitology   Check publisher's open access policy
ISSN 0020-7519
Publication date 1993-01-01
Sub-type Article (original research)
DOI 10.1016/0020-7519(93)90106-9
Open Access Status Not yet assessed
Volume 23
Issue 1
Start page 133
End page 136
Total pages 4
Subject 2405 Parasitology
2725 Infectious Diseases
Abstract Genetic differences among six didymozoid species distinguished on morphological criteria (Didymozoon species A, B, E, F and G, and Neometadidymozoon helicis) were sought by restriction fragment length analysis of internal transcribed spacer (ITS) and 5.8S ribosomal DNA. DNA fragments comprising ITS1, 5.8S and ITS2 were amplified by polymerase chain reaction for each of the six species. Digestion with the restriction enzyme (RE) Rsa I yielded four to six fragments, approximately 65-775 base pairs long. Digestion with another RE, Sau 3AI, yielded five to eight fragments, approximately 25-775 base pairs long. No intraspecific variation was detected but there were many interspecific differences. All six species distinguished by morphology are genetically distinct. Thus, the reliability of the morphological characters originally used to separate the species was confirmed.
Keyword 5.8S
Didymozoidae
Didymozoon
internal transcribed spacer
ITS
Neometadidymozoon helicis
PCR
polymerase chain reaction
rDNA
restriction fragment length analysis
ribosomal DNA
taxonomy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 28 Jun 2016, 15:41:34 EST by System User