Effects of ligand multivalency in binding studies: a general counterpart of the Scatchard analysis

Hogg P.J. and Winzor D.J. (1985) Effects of ligand multivalency in binding studies: a general counterpart of the Scatchard analysis. BBA - General Subjects, 843 3: 159-163. doi:10.1016/0304-4165(85)90134-5


Author Hogg P.J.
Winzor D.J.
Title Effects of ligand multivalency in binding studies: a general counterpart of the Scatchard analysis
Journal name BBA - General Subjects   Check publisher's open access policy
ISSN 0304-4165
Publication date 1985-12-13
Sub-type Article (original research)
DOI 10.1016/0304-4165(85)90134-5
Volume 843
Issue 3
Start page 159
End page 163
Total pages 5
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
2700 Medicine
Abstract A general counterpart of the Scatchard analysis has been developed which takes into account the valence of the ligand. Its use is first demonstrated by application to binding data obtained by exclusion chromatography of mixtures of Dextran T2000 and concanavalin A (a bivalent ligand) on a column of porous glass beads (Glyceryl-CPG 170) equilibrated at 5°C with phosphate-chloride buffer (pH 5.5), I 0.5. A recycling partition equilibrium study with Sephadex G-100 as gel phase then provides a quantitative evaluation of the interaction between haemoglobin and a monoclonal mouse antihaemoglobin antibody preparation in 0.1 M phosphate (pH 7.0) in order to emphasize the ability of the present analysis to consider collectively binding results obtained with a range of acceptor concentrations. Finally, the use of the generalized Scatchard analysis to assess acceptor site homogeneity is illustrated by reappraisal of results for the binding of glyceraldehyde-3-phosphate dehydrogenase to erythrocyte membranes.
Keyword Antigen-antibody interaction
Ligand multivalency
Membrane-receptor interaction
Scatchard analysis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 28 Jun 2016, 15:13:43 EST by System User