Expression of the genes coding for ornithine decarboxylase and its regulatory protein antizyme in the developing rat brain

Pujic Z., Matsumoto I. and Wilce P.A. (1995) Expression of the genes coding for ornithine decarboxylase and its regulatory protein antizyme in the developing rat brain. Developmental Neuroscience, 17 5-6: 286-291. doi:10.1159/000111298


Author Pujic Z.
Matsumoto I.
Wilce P.A.
Title Expression of the genes coding for ornithine decarboxylase and its regulatory protein antizyme in the developing rat brain
Journal name Developmental Neuroscience   Check publisher's open access policy
ISSN 1421-9859
Publication date 1995-01-01
Sub-type Article (original research)
DOI 10.1159/000111298
Volume 17
Issue 5-6
Start page 286
End page 291
Total pages 6
Subject 2806 Developmental Neuroscience
2808 Neurology
2800 Neuroscience
Abstract The ontogenic expression of the genes coding for ornithine decarboxylase (ODC) and its inhibitory protein, antizyme (AZ). was studied in the rat brain between embryonic day (E) 16 and postnatal day (P) 66. The level of ODC mRNA in whole brain was maximal at P2 and rapidly declined by P7 to a low level that was maintained into the adult. Levels of AZ mRNA also peaked at P2, and high levels were sustained into the adult. Regional studies indicated that between P2 and P60 ODC mRNA levels declined in the cerebral cortex, hippocampus and brainstem. These changes reflect the ontogenic pattern of protein levels and enzyme activity suggesting control of this enzyme may occur at the level of transcription. The level of AZ mRNA markedly increased in the cerebellum between P10 and P60. The level of ODC and AZ mRNA at P5 was not altered after continuous suppression of ODC enzyme activity by α-difluoromethylomithine between PO and P5. This suggests that ODC gene expression is not subject to product-related feedback inhibition during this period. Immunohistochemical localisation of ODC protein at P5 revealed ubiquitous distribution of immunoreactivity with higher levels in the hippocampus, cerebral cortex and discrete nuclei in the brainstem. ODC protein was undetectable in the adult rat brain.
Keyword Antizyme
CNS
Development
Gene expression
Ornithine decarboxylase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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