Effect of scrotal heating on testicular enzymes and spermatogenesis in the rat

Blackshaw A.W., Hamilton D. and Massey P.F. (1973) Effect of scrotal heating on testicular enzymes and spermatogenesis in the rat. Australian Journal of Biological Sciences, 26 6: 1395-1407. doi:10.1071/BI9731395


Author Blackshaw A.W.
Hamilton D.
Massey P.F.
Title Effect of scrotal heating on testicular enzymes and spermatogenesis in the rat
Journal name Australian Journal of Biological Sciences   Check publisher's open access policy
ISSN 0004-9417
Publication date 1973-01-01
Sub-type Article (original research)
DOI 10.1071/BI9731395
Volume 26
Issue 6
Start page 1395
End page 1407
Total pages 13
Subject 1103 Clinical Sciences
1309 Developmental Biology
1311 Genetics
1305 Biotechnology
1312 Molecular Biology
1310 Endocrinology
2743 Reproductive Medicine
2700 Medicine
Abstract Immersion of the scrotal testis of the rat in water at 42°C for 30 min caused rapid damage to pachytene primary spermatocytes in stages 1, 2, 3, and occasionally 8 of the seminiferous epithelium. There was cytoplasmic chromophilia with eosin or Alcian blue and binding of the fluorescent dye 2-p-toluidinylnapthalene-6-sulphonate to the cell membranes. Rapid dissolution of damaged cells occurred and most had disappeared by 28 hr after heating. Early round spermatids also showed nuclear changes with vacuolation and the development of “signet ring” nuclei. Quantitative estimates of germ cell damage showed that the pre-meiotic stages were most susceptible to heat and that the later stages showed mainly maturation depletion changes. Acid phosphatase and leucine aminopeptidase showed early histochemical increases about the damaged pachytene primary spermatocytes and these persisted until the damaged cells had been removed. Changes in the distribution of lactate and succinate dehydrogenases were also seen after heating.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 28 Jun 2016, 14:05:57 EST by System User