Effect of adjuvants on antibody responses of sheep immunised with recombinant pili from Dichelobacter nodosus

Walduck A.K. and Opdebeeck J.P. (1996) Effect of adjuvants on antibody responses of sheep immunised with recombinant pili from Dichelobacter nodosus. Australian Veterinary Journal, 74 6: 451-455.

Author Walduck A.K.
Opdebeeck J.P.
Title Effect of adjuvants on antibody responses of sheep immunised with recombinant pili from Dichelobacter nodosus
Journal name Australian Veterinary Journal   Check publisher's open access policy
ISSN 0005-0423
Publication date 1996-01-01
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 74
Issue 6
Start page 451
End page 455
Total pages 5
Subject 3400 Veterinary
Abstract The aroA locus of the Gram- pathogen Dichelobacter nodosus, which encodes 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, has been sequenced and expressed in Escherichia coli. The gene is located on a 1.48-kb DraI-HindIII fragment located directly upstream and in opposite transcriptional orientation to the gene encoding the fimbrial structural subunit. The deduced open reading frame is 1329 nucleotides in length, which encodes a protein of 443 amino acids (aa) with a calculated M(r) of 47,413, which was visualized in E. coli minicells, under the control of its native promoter. This derived aa sequence displays significant similarities with the sequences of the aroA gene products from a variety of microorganisms.
Keyword Adjuvants
DEAE-dextran
Humoral immunity
Incomplete Freunds adjuvant
Ovine footrot
Quil A
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 28 Jun 2016, 13:49:44 EST by System User