Anti-inflammatory activity of a lipid fraction (Lyprinol) from the NZ green-lipped mussel

Whitehouse M.W., Macrides T.A., Kalafatis N., Betts W.H., Haynes D.R. and Broadbent J. (1997) Anti-inflammatory activity of a lipid fraction (Lyprinol) from the NZ green-lipped mussel. Inflammopharmacology, 5 3: 237-246. doi:10.1007/s10787-997-0002-0

Author Whitehouse M.W.
Macrides T.A.
Kalafatis N.
Betts W.H.
Haynes D.R.
Broadbent J.
Title Anti-inflammatory activity of a lipid fraction (Lyprinol) from the NZ green-lipped mussel
Journal name Inflammopharmacology   Check publisher's open access policy
ISSN 0925-4692
Publication date 1997-01-01
Sub-type Article (original research)
DOI 10.1007/s10787-997-0002-0
Open Access Status Not yet assessed
Volume 5
Issue 3
Start page 237
End page 246
Total pages 10
Publisher Birkhauser Verlag AG
Language eng
Subject 2403 Immunology
3004 Pharmacology
Abstract A lipid-rich extract, preparared by supercritical fluid extraction of fresh stabilized mussel powder (Lyprinol), showed significant anti- inflammatory (AI) activity given therapeutically and prophylactically po to Wistar and Dark Agouti rats developing either (a) adjuvant-induced polyarthritis or (b) collagen(II)-induced autoallergic arthritis, with ED≤ 15 mg/kg; c.f. naproxen ≤ 25 mg/kg or various therapeutic oils (flaxseed, evening primrose, fish) ≤ 1800 mg/kg given orally. Lyprinol showed little or no activity in acute irritation assays (carrageenan, kaolin, histamine) indicating it is not mimicking rapid-acting NSAIDs. Incorporating Lyprinol into arthritigenic adjuvants composed of heat-killed Mycobacterium tuberculosis suspended in olive oil or squalane, effectively prevented arthritis development at a dose of 5 mg/rat. By contrast, 'dummy adjuvants' prepared with Mycobacterium tuberculosis and flaxseed, evening primrose or fish oils were still arthritigenic in Dark Agouti rats (doses of oil = 90 mg/rat). Lyprinol subfractions inhibited leukotriene-B biosynthesis by stimulated human polymorphonuclear leukocytes in vitro, and prostaglandin- E production by activated human macrophages in vitro. Much of this AI activity was associated with polyunsaturated fatty acids and natural antoxidants (carotenoids, etc.). In contrast to NSAIDs, Lyprinol is non- gastrotoxic in disease-stressed rats at 300 mg/kg po and does not seem to affect platelet aggregation (human, rat). These data show Lyprinol to be a reproducible, relatively stable, source of bioactive lipids with much greater potency than plant/marine oils currently used as nutritional supplements to ameliorate signs of inflammation.
Keyword Inflammation
Lipid fraction
NZ green-lipped mussel
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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