Isolation and cultivation of bovine ephemeral fever virus in chickens and chicken embryos

Elamin M.A.G. and Spradbrow P.B. (1978) Isolation and cultivation of bovine ephemeral fever virus in chickens and chicken embryos. Journal of Hygiene, 81 1: 1-7. doi:10.1017/S0022172400053705


Author Elamin M.A.G.
Spradbrow P.B.
Title Isolation and cultivation of bovine ephemeral fever virus in chickens and chicken embryos
Journal name Journal of Hygiene   Check publisher's open access policy
ISSN 0022-1724
Publication date 1978-01-01
Sub-type Article (original research)
DOI 10.1017/S0022172400053705
Volume 81
Issue 1
Start page 1
End page 7
Total pages 7
Subject 2739 Public Health, Environmental and Occupational Health
2403 Immunology
Abstract Unadapted bovine ephemeral fever (BEF) virus was isolated from cattle blood after intravenous inoculation into chicken embryos. Infected embryos died or hatched as abnormal chickens. The chick embryo was slightly less sensitive to unadapted BEF virus than were Vero cell cultures, but the use of embryos avoids the several blind passages that are required to isolate BEF virus in unweaned mice. Chick embryos were considerably less efficient than Vero cell culture or unweaned mice in detecting Vero cell-adapted and mouse-adapted BEF virus respectively.Viraemia was demonstrated in chicken embryos at 1–4 days and in one-day-old chickens at 1–3 days after intravenous inoculation of BEF virus. BEF virus was demonstrated by isolation and by immunofluoresence in heart, brain, lung and liver of chicken embryos at 1–5 days and in lung and liver of one-day-old chickens at 1–2 days, after intravenous inoculation. The isolated viruses were confirmed as BEF virus by neutralization with immune mouse ascitic fluid. BEF neutralizing antibodies were produced in 4-week-old and adult chickens after intravenous inoculation with BEF virus.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 28 Jun 2016, 13:29:32 EST by System User