Quantitative characterization of the interactions of aldolase and glyceraldehyde-3-phosphate dehydrogenase with erythrocyte membranes

Kelley G.E. and Winzor D.J. (1984) Quantitative characterization of the interactions of aldolase and glyceraldehyde-3-phosphate dehydrogenase with erythrocyte membranes. BBA - Biomembranes, 778 1: 67-73. doi:10.1016/0005-2736(84)90448-6


Author Kelley G.E.
Winzor D.J.
Title Quantitative characterization of the interactions of aldolase and glyceraldehyde-3-phosphate dehydrogenase with erythrocyte membranes
Journal name BBA - Biomembranes   Check publisher's open access policy
ISSN 0005-2736
Publication date 1984-11-21
Sub-type Article (original research)
DOI 10.1016/0005-2736(84)90448-6
Volume 778
Issue 1
Start page 67
End page 73
Total pages 7
Subject 1303 Specialist Studies in Education
1304 Biophysics
1307 Cell Biology
Abstract Results of studies on the interactions of aldolase and glyceraldehyde-3-phosphate dehydrogenase with erythrocyte ghosts have been reinterpreted by making allowance for possible multivalency of the enzymes in regard to their interactions with matrix sites. It is shown that the curvilinearity of the experimental Scatchard plots may be attributed fully to the formation of enzyme-membrane complexes in which tetravalent enzyme may form crosslinks between several membrane sites. This interpretation of the results is preferable to earlier analyses based on heterogeneity of membrane sites in that (a) it takes into account the tetrameric nature of aldolase and glyceraldehyde-3-phosphate dehydrogenase, and (b) it is consistent with experimental demonstrations that band 3 protein is the sole site for enzyme interaction with the erythrocyte matrix. The dependence on ionic strength of the intrinsic association constant for either interaction is such that the binding of neither aldolase nor glyceraldehyde-3-phosphate dehydrogenase could be detected at ionic strengths in excess of 0.08 I. This finding is discussed in relation to the claims and counterclaims concerning the physiological significance of these interactions between glycolytic enzymes and erythrocyte membranes.
Keyword (Erythrocyte membrane)
Aldolase
Band 3
Binding site
Glyceraldehyde-3-phosphate dehydrogenase
Membrane-protein interaction
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 28 Jun 2016, 12:47:10 EST by System User