Decay of granulocyte adherence following isolation from human blood

Kelly M.K. and Thong Y.H. (1984) Decay of granulocyte adherence following isolation from human blood. Immunology Letters, 7 6: 309-313. doi:10.1016/0165-2478(84)90086-5


Author Kelly M.K.
Thong Y.H.
Title Decay of granulocyte adherence following isolation from human blood
Journal name Immunology Letters   Check publisher's open access policy
ISSN 0165-2478
Publication date 1984-01-01
Sub-type Article (original research)
DOI 10.1016/0165-2478(84)90086-5
Volume 7
Issue 6
Start page 309
End page 313
Total pages 5
Subject 2403 Immunology
2723 Immunology and Allergy
Abstract We studied the rate of decay of granulocyte adherence following isolation from human blood using the nylon-fibre microcolumn technique recently developed in our laboratory. The decay in adherence was found to be evident by 6 h, pronounced by 24 h, and very substantial by 48 h. This decay in adherence can be reversed by incubation at 4 °C instead of 37 °C. It can also be retarded by incubation with both superoxide dismutase and 2,3-dihydroxybenzoic acid. The data suggests that peroxidation of the granulocyte membrane is a possible mechanism for the decay in granulocyte adherence. These results provide further insight into biological aspects of granulocyte activity, and have implications for the laboratory evaluation of granulocyte function.
Keyword adherence
auto-oxidation
chemotaxis
granulocytes
metabolism
neutrophils
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 28 Jun 2016, 12:07:41 EST by System User