Characterization of glutamine uptake in mouse two-cell embryos and blastocysts

Lewis A.M. and Kaye P.L. (1992) Characterization of glutamine uptake in mouse two-cell embryos and blastocysts. Journal of Reproduction and Fertility, 95 1: 221-229. doi:10.1530/jrf.0.0950221


Author Lewis A.M.
Kaye P.L.
Title Characterization of glutamine uptake in mouse two-cell embryos and blastocysts
Journal name Journal of Reproduction and Fertility   Check publisher's open access policy
ISSN 0022-4251
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1530/jrf.0.0950221
Open Access Status Not yet assessed
Volume 95
Issue 1
Start page 221
End page 229
Total pages 9
Language eng
Subject 1309 Developmental Biology
1312 Molecular Biology
1314 Physiology
2710 Embryology
2729 Obstetrics and Gynaecology
Abstract Mouse two-cell embryos and blastocysts take up [H]glutamine in vitro at a constant rate for at least 15 min, depending on the concentration of glutamine and developmental stage of the embryo. Uptake by two-cell embryos can be resolved into two saturable components. The major contributing system is Na independent, inhibited by alanine, methionine, 2-amino-2-norbornanecarboxylic acid (BCH) or leucine and has a K(m) of 3856 ± 672 μmol l and V(max) of 436 ± 58 fmol per embryo per 10 min. These features are characteristics of the ubiquitous system L transporter. The second component is Na dependent with K(m) of 1064 ± 914 μmol l and V(max) 107 ± 47 fmol per embryo per 10 min. Similar V(max) and inhibition of this component by glycine suggest a low reactivity with the gly-system. Blastocyst uptake of glutamine is mainly by a Na-dependent saturable mechanism with K(m) of 524 ± 75 μmol l and V(max) of 1264 ± 101 fmol per embryo per 10 min which is inhibited by alanine, isoleucine, leucine and BCH, features characteristic of the system B. The increase in uptake capacity as a consequence of the appearance of the system B may be related to increased metabolic requirements for glutamine, in the rapidly expanding blastocyst.
Keyword Blastocyst
Embryo
Glutamine transport
Mouse
System B0.+
System L
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 28 Jun 2016, 11:48:31 EST by System User