Allowance for "analyte valence" in the retention model of ion-exchange chromatography. Studies of adenosine 5′-phosphates on deae cellulose

Bergman D.A. and Winzor D.J. (1987) Allowance for "analyte valence" in the retention model of ion-exchange chromatography. Studies of adenosine 5′-phosphates on deae cellulose. Journal of Chromatography A, 391 C: 67-77. doi:10.1016/S0021-9673(01)94305-5


Author Bergman D.A.
Winzor D.J.
Title Allowance for "analyte valence" in the retention model of ion-exchange chromatography. Studies of adenosine 5′-phosphates on deae cellulose
Journal name Journal of Chromatography A   Check publisher's open access policy
ISSN 0021-9673
Publication date 1987-01-01
Sub-type Article (original research)
DOI 10.1016/S0021-9673(01)94305-5
Open Access Status Not yet assessed
Volume 391
Issue C
Start page 67
End page 77
Total pages 11
Subject 1602 Criminology
1308 Clinical Biochemistry
1313 Molecular Medicine
Abstract Quantitative expressions are derived for various retention models of the ion-exchange behaviour of multivalent analytes. Their effectiveness as descriptions of experimental results is then tested by application to partition equilibrium studies of the effect of phosphate concentration on the interaction between the three adenosine 5′-phosphates and diethylaminoethylcellulose at pH 4.4. A completely general multi-state model has pointed to quantitative deficiencies in the currently accepted cooperative two-state model, which is, however, superior to a multi-state model devoid of cooperative effects. A similar situation pertains to the ion-exchange behaviour of cytochrome c on carboxymethylcellulose at pH 7.0.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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