Neuronal cell lines as model dorsal root ganglion neurons: a transcriptomic comparison

Yin, Kathleen, Baillie, Gregory J. and Vetter, Irina (2016) Neuronal cell lines as model dorsal root ganglion neurons: a transcriptomic comparison. Molecular Pain, 12 1-17. doi:10.1177/1744806916646111


Author Yin, Kathleen
Baillie, Gregory J.
Vetter, Irina
Title Neuronal cell lines as model dorsal root ganglion neurons: a transcriptomic comparison
Journal name Molecular Pain   Check publisher's open access policy
ISSN 1744-8069
Publication date 2016-04-01
Year available 2016
Sub-type Article (original research)
DOI 10.1177/1744806916646111
Open Access Status DOI
Volume 12
Start page 1
End page 17
Total pages 17
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Abstract Background: Dorsal root ganglion neuron-derived immortal cell lines including ND7/23 and F-11 cells have been used extensively as in vitro model systems of native peripheral sensory neurons. However, while it is clear that some sensory neuron-specific receptors and ion channels are present in these cell lines, a systematic comparison of the molecular targets expressed by these cell lines with those expressed in intact peripheral neurons is lacking.
Formatted abstract
Background Dorsal root ganglion neuron-derived immortal cell lines including ND7/23 and F-11 cells have been used extensively as in vitro model systems of native peripheral sensory neurons. However, while it is clear that some sensory neuron-specific receptors and ion channels are present in these cell lines, a systematic comparison of the molecular targets expressed by these cell lines with those expressed in intact peripheral neurons is lacking.

Results In this study, we examined the expression of RNA transcripts in the human neuroblastoma-derived cell line, SH-SY5Y, and two dorsal root ganglion hybridoma cell lines, F-11 and ND7/23, using Illumina next-generation sequencing, and compared the results with native whole murine dorsal root ganglions. The gene expression profiles of these three cell lines did not resemble any specific defined dorsal root ganglion subclass. The cell lines lacked many markers for nociceptive sensory neurons, such as the Transient receptor potential V1 gene, but expressed markers for both myelinated and unmyelinated neurons. Global gene ontology analysis on whole dorsal root ganglions and cell lines showed similar enrichment of biological process terms across all samples.

Conclusions This paper provides insights into the receptor repertoire expressed in common dorsal root ganglion neuron-derived cell lines compared with whole murine dorsal root ganglions, and illustrates the limits and potentials of these cell lines as tools for neuropharmacological exploration.
Keyword Dorsal root ganglions
SH-SY5Y
F-11
ND7/23
Transcriptomic analysis
Molecular expression
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Pharmacy Publications
Institute for Molecular Bioscience - Publications
 
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