Determining multiple responses of Pseudomonas aeruginosa PAO1 to an antimicrobial agent, free nitrous acid

Gao, Shu-Hong, Fan, Lu, Peng, Lai, Guo, Jianhua, Agullo-Barcelo, Miriam, Yuan, Zhiguo and Bond, Philip L. (2016) Determining multiple responses of Pseudomonas aeruginosa PAO1 to an antimicrobial agent, free nitrous acid. Environmental Science and Technology, 50 10: 5305-5312. doi:10.1021/acs.est.6b00288


Author Gao, Shu-Hong
Fan, Lu
Peng, Lai
Guo, Jianhua
Agullo-Barcelo, Miriam
Yuan, Zhiguo
Bond, Philip L.
Title Determining multiple responses of Pseudomonas aeruginosa PAO1 to an antimicrobial agent, free nitrous acid
Formatted title
Determining multiple responses of Pseudomonas aeruginosa PAO1 to an antimicrobial agent, free nitrous acid
Journal name Environmental Science and Technology   Check publisher's open access policy
ISSN 1520-5851
0013-936X
Publication date 2016-05-17
Year available 2016
Sub-type Article (original research)
DOI 10.1021/acs.est.6b00288
Open Access Status Not yet assessed
Volume 50
Issue 10
Start page 5305
End page 5312
Total pages 8
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Formatted abstract
Free nitrous acid (FNA) has recently been demonstrated as an antimicrobial agent on a range of micro-organisms, especially in wastewater-treatment systems. However, the antimicrobial mechanism of FNA is largely unknown. Here, we report that the antimicrobial effects of FNA are multitargeted. The response of a model denitrifier, Pseudomnas aeruginosa PAO1 (PAO1), common in wastewater treatment, was investigated in the absence and presence of inhibitory level of FNA (0.1 mg N/L) under anaerobic denitrifying conditions. This was achieved through coupling gene expression analysis, by RNA sequencing, and with a suite of physiological analyses. Various transcripts exhibited significant changes in abundance in the presence of FNA. Respiration was likely inhibited because denitrification activity was severely depleted, and decreased transcript levels of most denitrification genes occurred. As a consequence, the tricarboxylic acid (TCA) cycle was inhibited due to the lowered cellular redox state in the FNA-exposed cultures. Meanwhile, during FNA exposure, PAO1 rerouted its carbon metabolic pathway from the TCA cycle to pyruvate fermentation with acetate as the end product as a possible survival mechanism. Additionally, protein synthesis was significantly decreased, and ribosome preservation was evident. These findings improve our understanding of PAO1 in response to FNA and contribute toward the potential application for use of FNA as an antimicrobial agent.
Keyword General Chemistry
Environmental Chemistry
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID DP120102832
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Advanced Water Management Centre Publications
 
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