Cytoarchitecture, neuronal composition, and entorhinal afferents of the flying fox hippocampus.

Buhl E.H. and Dann J.F. (1991) Cytoarchitecture, neuronal composition, and entorhinal afferents of the flying fox hippocampus.. Hippocampus, 1 2: 131-152. doi:10.1002/hipo.450010203

Author Buhl E.H.
Dann J.F.
Title Cytoarchitecture, neuronal composition, and entorhinal afferents of the flying fox hippocampus.
Journal name Hippocampus   Check publisher's open access policy
ISSN 1050-9631
Publication date 1991-01-01
Sub-type Article (original research)
DOI 10.1002/hipo.450010203
Volume 1
Issue 2
Start page 131
End page 152
Total pages 22
Language eng
Subject 2800 Neuroscience
Abstract In a comparative approach, the anatomical organization of the hippocampus was investigated in two species of megachiropteran bats, the grey‐headed flying fox, Pteropus poliocephalus, and the little red flying fox,Pteropus scapulatus. In general, the cytoarchitectonic appearance of the flying fox hippocampus corresponded well with that of other mammals, revealing all major subdivisions. While the dentate fascia was trilaminated with a molecular layer, a granule cell layer, and a distinct polymorphic layer, the ammonic subfields were subdivided into stratum lacunosum moleculare, stratum radiatum, stratum lucidum or mossy fiber layer (restricted to the CA3 region), pyramidal cell layer, and stratum oriens. In Ammon's horn, only subfields CA1, CA3, and CA3c were clearly discernible, whereas the CA2 region remained indistinct. In some cytoarchitectonic features, such as the dispersion of the pyramidal layer in CA1, the megachiropteran hippocampus resembled the corresponding region in primates. Five characteristic neuronal cell types of the megachiropteran hippocampus were studied in fixed slice preparations after intracellular injection with Lucifer Yellow. While the morphological appearance of CA3 pyramidal cells, horizontal stratum oriens cells, aspiny stellate cells, and mossy cells strongly resembled their counterparts in rodents, primates, and carnivores, granule cells showed an interesting variation from the nonprimate pattern. Like a subset of granule cells in the primate dentate gyrus, 75% of flying fox granule cells revealed 1–2 basal dendrites that ramified in the polymorphic layer. These processes are presumed to form the morphological substrate for recurrent excitation. Entorhinal afferents to Ammon's horn and the dentate fascia were revealed by employing the method of tract tracing in fixed tissue with the carbocyanine dye Dil. Similar to the rat and cat, but unlike the monkey, the entorhino‐dentate projection in the flying fox is bilaminate, with medial entorhinal afferents occupying the middle third of the molecular layer and lateral entorhinal axons ramifying closer to the hippocampal fissure. The remaining inner third of the molecular layer was free from entorhinal input. In contrast to the radial organization of the projection to dentate gyrus and subfield CA3, entorhinal afferents to region CA1 followed a proximo‐distal gradient, with medial entorhinal afferents terminating closer to the CA3/CA1 border. Photoconverted preparations were used to determine the trajectory of individual axons. The majority of entorhino‐dentate axons traversed the hippocampal fissure, usually close to the crest region, and gave rise to several terminal branches with numerous en passant varicosities. Individual fibers coursed for considerable distances parallel to the granule cell layer, thus presumably activating a large number of postsynaptic granule cells. It is concluded that in several features of its anatomical organization, the flying fox hippocampus corresponds with that of primates. Therefore, these findings corroborate previous evidence that megachiropteran bats have evolved from an early branch of the primate lineage. Copyright
Keyword anterograde tracing
fixed slices
intracellular injection
neuronal morphology
perforant path
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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Citation counts: Scopus Citation Count Cited 27 times in Scopus Article | Citations
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Created: Tue, 14 Jun 2016, 16:22:51 EST by System User