The effect of various adjuvants on the humoral immune response of sheep and cattle to soluble and membrane midgut antigens of Boophilus microplus

Jackson L.A. and Opdebeeck J.P. (1995) The effect of various adjuvants on the humoral immune response of sheep and cattle to soluble and membrane midgut antigens of Boophilus microplus. Veterinary Parasitology, 58 1-2: 129-141. doi:10.1016/0304-4017(94)00703-F


Author Jackson L.A.
Opdebeeck J.P.
Title The effect of various adjuvants on the humoral immune response of sheep and cattle to soluble and membrane midgut antigens of Boophilus microplus
Journal name Veterinary Parasitology   Check publisher's open access policy
ISSN 0304-4017
Publication date 1995-01-01
Sub-type Article (original research)
DOI 10.1016/0304-4017(94)00703-F
Volume 58
Issue 1-2
Start page 129
End page 141
Total pages 13
Subject 2405 Parasitology
3400 Veterinary
1103 Clinical Sciences
Abstract Sheep and cattle were immunized with soluble and membrane midgut antigens of the cattle tick, Boophilus microplus in association with various adjuvants and antibody levels to soluble and membrane midgut extracts were measured by enzyme-linked immunosorbent assay (ELISA). The adjuvants used were Quil A, Freund's incomplete adjuvant (FIA) and aluminium hydroxide (Al(OH)3). Significant antibody levels to midgut membrane antigen (GM) were not detected in the sera of sheep immunized with GM plus Al(OH)3. However, membrane and soluble midgut antigens combined with Quil A generated significantly higher levels of antibodies in sheep than these antigens emulsified in FIA (P < 0.05). In cattle, although soluble midgut antigen (GS) plus Quil A induced significantly higher levels of antibodies compared with GS in FIA (P < 0.05), neither preparation provided significant protective immunity against challenge with B. microplus (46% and 22%, respectively). A soluble form of membrane midgut antigen extracted by low ionic strength buffer (LI-GM) when combined with Quil A provided 83% and 87% protection against challenge with B. microplus in two separate experiments. Quil A was clearly superior to FIA and Al(OH)3 as an adjuvant for these tick antigens.
Keyword Adjuvants
Boophilus microplus
Cattle-Arthropoda
Immune response-Arthropoda
Midgut antigens
Sheep-Arthropoda
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
 
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