Human gingival Langerhans cells as accessory cells in mitogen induced T cell responses.

Newcomb G.M. and Powell R.N. (1988) Human gingival Langerhans cells as accessory cells in mitogen induced T cell responses.. Journal of Periodontology, 59 12: 811-815. doi:10.1902/jop.1988.59.12.811


Author Newcomb G.M.
Powell R.N.
Title Human gingival Langerhans cells as accessory cells in mitogen induced T cell responses.
Journal name Journal of Periodontology   Check publisher's open access policy
ISSN 0022-3492
Publication date 1988-01-01
Sub-type Article (original research)
DOI 10.1902/jop.1988.59.12.811
Open Access Status Not yet assessed
Volume 59
Issue 12
Start page 811
End page 815
Total pages 5
Language eng
Subject 3500 Dentistry
Abstract Peripheral blood and gingival tissue was collected from patients undergoing surgery for the treatment of periodontal disease. Both unfractionated peripheral blood mononuclear cells and a T cell-enriched, monocyte-depleted lymphocyte population were isolated from the blood samples while a gingival epithelial cell suspension was prepared from the oral aspect of the gingival tissue. The blood cells (1 X 10(5)) were then co-cultured with the gingival epithelial cells (1 X 10(5) and 1 X 10(4)) in 200 microliters aliquots in microtitre trays for three days to see if the epithelial cells could act as accessory cells in the lymphocyte response to the mitogen, phytohaemagglutinin (PHA). Lymphocyte proliferation was assessed by measuring the uptake of [6-3H] thymidine during the last 18 hours of culture. Removal of monocytes from peripheral blood mononuclear cells led to a significant, but not total, reduction in the proliferative response to PHA. When gingival epithelial cells were added to the T cells they restored their response to PHA in a dose dependent fashion. As Langerhans cells were the only potentially immunocompetent cells seen in the epithelial cell suspensions, it is concluded that these cells are acting as accessory cells in the T cell response to PHA.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 14 Jun 2016, 15:23:15 EST by System User