Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases

Hugenholtz, P and Huber, T (2003) Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases. International Journal of Systematic And Evolutionary Microbiology, 53 1: 289-293. doi:10.1099/ijs.0.02441-0


Author Hugenholtz, P
Huber, T
Title Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases
Journal name International Journal of Systematic And Evolutionary Microbiology   Check publisher's open access policy
ISSN 1466-5026
Publication date 2003-01-01
Year available 2003
Sub-type Article (original research)
DOI 10.1099/ijs.0.02441-0
Open Access Status Not yet assessed
Volume 53
Issue 1
Start page 289
End page 293
Total pages 5
Place of publication Reading, England
Publisher Society for General Microbiology
Language eng
Abstract A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.
Keyword Microbiology
Ribosomal-rna Genes
Microbial Diversity
Bacterial Diversity
Pcr Coamplification
Hydrothermal Vent
Amplification
Populations
Consequence
Sediments
Frequency
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Citation counts: TR Web of Science Citation Count  Cited 151 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 13 Aug 2007, 23:25:37 EST