The use of guinea pig K+-depolarized tracheal chain preparations in β-adrenoceptor studies

O'Donnell S.R. and Wanstall J.C. (1980) The use of guinea pig K+-depolarized tracheal chain preparations in β-adrenoceptor studies. Journal of Pharmacological Methods, 4 1: 43-50. doi:10.1016/0160-5402(80)90042-X


Author O'Donnell S.R.
Wanstall J.C.
Title The use of guinea pig K+-depolarized tracheal chain preparations in β-adrenoceptor studies
Journal name Journal of Pharmacological Methods   Check publisher's open access policy
ISSN 0160-5402
Publication date 1980-01-01
Sub-type Article (original research)
DOI 10.1016/0160-5402(80)90042-X
Open Access Status Not yet assessed
Volume 4
Issue 1
Start page 43
End page 50
Total pages 8
Language eng
Subject 3004 Pharmacology
Abstract Guinea pig tracheal chain preparations were K-depolarized using Krebs solution in which all the Na was replaced by an equivalent amount of K. This caused a sustained contraction of the preparations. Reproducible concentration-response (relaxation) curves to isoprenaline could be obtained provided that the preparations were repolarized by washing in normal Krebs solution between curves. pA values for propranolol (8.30) and butoxamine (5.89) were in good agreement with values obtained on other types of tracheal preparation. The maximum relaxation to salbutamol was less than that to isoprenaline and the maxima to both drugs were less than the maximum relaxation of the tissue (to papaverine). It is suggested that K-depolarized tracheal preparations have no β-adrenoceptor reserve and may, therefore, be useful in studies designed to compare the efficacies of β-adrenoceptor agonists.
Keyword Guinea pig tracheal chain
K-depolarization
β-adrenoceptor agonists
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 14 Jun 2016, 14:09:46 EST by System User