Early cellular events in an actinomycin D-created dentin niche in the rat incisor

Moule A.J., Young W.G. and Adkins K.F. (1993) Early cellular events in an actinomycin D-created dentin niche in the rat incisor. Journal of Oral Pathology and Medicine, 22 4: 159-167. doi:10.1111/j.1600-0714.1993.tb01050.x


Author Moule A.J.
Young W.G.
Adkins K.F.
Title Early cellular events in an actinomycin D-created dentin niche in the rat incisor
Journal name Journal of Oral Pathology and Medicine   Check publisher's open access policy
ISSN 0904-2512
Publication date 1993-01-01
Year available 1993
Sub-type Article (original research)
DOI 10.1111/j.1600-0714.1993.tb01050.x
Open Access Status Not yet assessed
Volume 22
Issue 4
Start page 159
End page 167
Total pages 9
Place of publication COPENHAGEN
Publisher MUNKSGAARD INT PUBL LTD
Language eng
Subject 1306 Cancer Research
2734 Pathology and Forensic Medicine
3500 Dentistry
Abstract Administration of actinomycin D at a dose level of 0.375 mug/g resulted in the selective disruption of developing odontoblasts at a critical stage of morphogenesis. A dentin niche was formed which was later repaired by cellular reparative dentin. The cellular changes which resulted in dentin niche formation were studied histologically and ultrastructurally in serial longitudinal and transverse sections from tissues obtained 10 h to 80 h following injection of the drug. Five stages were identified: initial destruction (10-20 h), rapid destruction (30-40 h), debris removal (50-60 h), proliferation (60-80 h) and matrix deposition (post 80 h). The cellular changes found in the dental papilla were considerably different from those found in inflammation, resolution and repair of fibrous connective tissue. These early stages were dominated by apoptosis and heterophagy, and after 80 h by disordered dentin matrix formation. The three-dimensional morphology of the defect was reconstructed from serial sections. The shape of the niche was the result of interference by actinomycin D in the patterns of proliferation and migration of the cells in the apical region of the rat incisor tooth.
Keyword Mesenchymal Cells
Death
Doxorubicin
Populations
Renewal
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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