Effect of growth rate on stability of a recombinant plasmid during continuous culture of Saccharomyces cerevisiae in non-selective medium

Impoolsup A., Caunt P. and Greenfield P.F. (1989) Effect of growth rate on stability of a recombinant plasmid during continuous culture of Saccharomyces cerevisiae in non-selective medium. Journal of Biotechnology, 10 2: 171-180. doi:10.1016/0168-1656(89)90039-4


Author Impoolsup A.
Caunt P.
Greenfield P.F.
Title Effect of growth rate on stability of a recombinant plasmid during continuous culture of Saccharomyces cerevisiae in non-selective medium
Journal name Journal of Biotechnology   Check publisher's open access policy
ISSN 0168-1656
Publication date 1989-01-01
Sub-type Article (original research)
DOI 10.1016/0168-1656(89)90039-4
Open Access Status Not yet assessed
Volume 10
Issue 2
Start page 171
End page 180
Total pages 10
Subject 1305 Biotechnology
Abstract The stability of a 2μm based plasmid, pLG669-z, was measured in a Saccharomyces cerevisiae strain, grown continuously in a non-selective medium. The apparent stability of the plasmid was found to decrease with increasing growth rate. A model was developed to predict the contributions of segregational instability and growth rate differences to the apparent stability. An increase in segregational instability was the dominant factor in the increased rate of plasmid loss.
Keyword Growth rate
Non-selective medium
Recombinant plasmid
Saccharomyces cerevisiae
Stability
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 14 Jun 2016, 12:37:10 EST by System User