Toxins from the box-jellyfish Chironex fleckeri

Endean R., Monks S.A. and Cameron A.M. (1993) Toxins from the box-jellyfish Chironex fleckeri. Toxicon, 31 4: 397-410. doi:10.1016/0041-0101(93)90175-I

Author Endean R.
Monks S.A.
Cameron A.M.
Title Toxins from the box-jellyfish Chironex fleckeri
Journal name Toxicon   Check publisher's open access policy
ISSN 0041-0101
Publication date 1993-01-01
Sub-type Article (original research)
DOI 10.1016/0041-0101(93)90175-I
Volume 31
Issue 4
Start page 397
End page 410
Total pages 14
Language eng
Subject 3005 Toxicology
Abstract Two myotoxins (T1 and T2) with mol. wts of approximately 600,000 and 150,000, respectively, and a haemolysin (T3) with a mol. wt of approximately 70,000 were isolated from the crude nematocyst venom of C. fleckeri by the use of Sephadex G-200 chromatography. A neurotoxic fraction (T4) and a haemolytic fraction (T5) containing proteins with apparent mol. wts of approximately 150,000 and 70,000, respectively, were also isolated by Sephadex chromatography from crude extracts of tentacular material from which nematocysts had been removed. The three nematocyst toxins and the two toxic fractions from tentacle extracts were lethal to mice on i.v. injection. After SDS-PAGE the myotoxins T1 and T2 yielded similar major bands corresponding with mol. wts different from those yielded by T3 and the toxic tentacle fractions. T1 and T2 appeared to be comprised of aggregations of subunits with mol. wts of approximately 18,000. On HPLC, crude nematocyst venom and the nematocyst toxins T1 and T2 lost their myotoxic properties. The need for thorough removal of extraneous tentacular material from isolated nematocysts, the need for effective rupture of nematocysts, the need to counter the lability of the nematocyst venom and the need to use myotoxicity as a criterion of venom activity if the active components of the venom are to be purified and characterized are emphasized.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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Citation counts: TR Web of Science Citation Count  Cited 40 times in Thomson Reuters Web of Science Article | Citations
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