On the distribution of aldolase isoenzymes in subcellular fractions from rat brain

Clarke F.M. and Masters C.J. (1973) On the distribution of aldolase isoenzymes in subcellular fractions from rat brain. Archives of Biochemistry and Biophysics, 156 2: 673-683. doi:10.1016/0003-9861(73)90320-2

Author Clarke F.M.
Masters C.J.
Title On the distribution of aldolase isoenzymes in subcellular fractions from rat brain
Journal name Archives of Biochemistry and Biophysics   Check publisher's open access policy
ISSN 1096-0384
Publication date 1973-01-01
Sub-type Article (original research)
DOI 10.1016/0003-9861(73)90320-2
Volume 156
Issue 2
Start page 673
End page 683
Total pages 11
Language eng
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract As an extension of previous studies on the adsorption of aldolase (EC in nervous tissue, the main features of the subcellular localization of this enzyme in rat brain have been investigated. The major portion of the aldolase activity in homogenates of this tissue was demonstrated to be present in association with the particulate material, and a differential distribution of the AC isoenzymes was evident between the membranes and the cytosol. Some of the enzyme which was associated with the particulate fraction was shown to be occluded rather than absorbed to the membranes. This type of association was evident in the nuclear and mitochondrial fractions, in particular, with the occluded enzyme presenting an isoenzyme content high in C-type activity, and similar to that of the cytosol. The microsomal fraction contained a high proportion of enzyme in the bound form. Isoenzyme analysis of the enzyme in this microsomal fraction revealed a preferential association between the particulate material and A-type aldolase activity. A purified membrane fraction was prepared from the primary microsomal fraction, and identified as the main site of aldolase binding. The significance of the differential binding of aldolase isoenzymes and its localization amongst the subcellular fractions of rat brain have been discussed in relation to the structural and metabolic features of this tissue, and the coupling of energy producing sequences with energy requiring processes.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
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