Extracellular ascorbic acid in lung

Willis R.J. and Kratzing C.C. (1976) Extracellular ascorbic acid in lung. BBA - General Subjects, 444 1: 108-117. doi:10.1016/0304-4165(76)90228-2


Author Willis R.J.
Kratzing C.C.
Title Extracellular ascorbic acid in lung
Journal name BBA - General Subjects   Check publisher's open access policy
ISSN 0304-4165
Publication date 1976-08-24
Sub-type Article (original research)
DOI 10.1016/0304-4165(76)90228-2
Volume 444
Issue 1
Start page 108
End page 117
Total pages 10
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
2700 Medicine
Abstract Fifty percent of the ascorbic acid content of sliced rat lung was released from tissue to the media within a few minutes by either washing or incubating the slices with Kreb-phosphate solution. Measurement of the lactate dehydrogenase and potassium content of the medium after incubating lung slices for 5 min showed that about 20% of the cells were damaged by slicing. Sephadex chromatography of tissue extracts prepared from washed lung slices showed that none of the ascorbic acid in these slices was bound to protein. Also, metabolic poisons were shown to deplete the ascorbic acid content of washed lung slices. Approx. 57% of the lung ascorbic acid of guinea pigs that had been supplemented with ascorbic acid and 78% of the lung ascorbic acid of ascorbic acid-deficient guinea pigs were found in the medium when lung slices from these animals were incubated with Krebs-phosphate solution. These results were taken to indicate the presence of an extracellular pool of ascorbic acid in lung which is maintained even during scurvy.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 14 Jun 2016, 12:13:21 EST by System User