Photochromic intensification of diaminobenzidine reaction product in the presence of tetrazolium salts: applications for intracellular labelling and immunohistochemistry

Vaney D.I. (1992) Photochromic intensification of diaminobenzidine reaction product in the presence of tetrazolium salts: applications for intracellular labelling and immunohistochemistry. Journal of Neuroscience Methods, 44 2-3: 217-223. doi:10.1016/0165-0270(92)90013-4


Author Vaney D.I.
Title Photochromic intensification of diaminobenzidine reaction product in the presence of tetrazolium salts: applications for intracellular labelling and immunohistochemistry
Journal name Journal of Neuroscience Methods   Check publisher's open access policy
ISSN 0165-0270
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1016/0165-0270(92)90013-4
Open Access Status Not Open Access
Volume 44
Issue 2-3
Start page 217
End page 223
Total pages 7
Subject 2800 Neuroscience
Abstract The diaminobenzidine (DAB) reaction product can be greatly intensified by incubating the reacted tissue in either nitro blue tetrazolium or tetranitro blue tetrazolium and then exposing the tissue to strong light. Epi-illumination through a microscope objective enables the photochromic intensification to be carried out under direct visual control, with optimal intensification taking only 10-30 s through a 20 × objective. Alternatively, the whole preparation can be intensified in a few minutes by passing it back and forth under a fibre light guide. The method can be used to intensify cells that have been labelled either by immunoperoxidase techniques or with intracellular tracers such as horseradish peroxidase and neurobiotin.
Keyword Diaminobenzidine
Immunohistochemistry
Neurobiotin
Photochromic intensification
Retina
Tetrazolium salts
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 14 Jun 2016, 12:05:22 EST by System User