A cholesterol implant used as a delivery system to immunize mice with bovine serum albumin

Opdebeeck J.P. and Tucker I.G. (1993) A cholesterol implant used as a delivery system to immunize mice with bovine serum albumin. Journal of Controlled Release, 23 3: 271-279. doi:10.1016/0168-3659(93)90008-S


Author Opdebeeck J.P.
Tucker I.G.
Title A cholesterol implant used as a delivery system to immunize mice with bovine serum albumin
Journal name Journal of Controlled Release   Check publisher's open access policy
ISSN 0168-3659
Publication date 1993-01-01
Sub-type Article (original research)
DOI 10.1016/0168-3659(93)90008-S
Open Access Status Not yet assessed
Volume 23
Issue 3
Start page 271
End page 279
Total pages 9
Subject 3003 Pharmaceutical Science
Abstract Bovine serum albumin (BSA; 3 mg) compressed into 30 mg cholesterol pellets and implanted under the skin of mice for 77 days was delivered (> 90%) in an initial burst in the first 6 days after implantation and slowly thereafter. Antigen delivered thus from the cholesterol pellet was more effective in inducing anti-BSA antibody levels in mice than either a single injection or 3 injections of the same dose of BSA (P < 0.05). Injection of BSA subcutaneously in the region of blank cholesterol pellets showed the delivery profile rather than the presence of pellets was responsible for the adjuvant-like effect. Decreasing the dose of BSA (3, 1, 0.5 and 0.15 mg) in the cholesterol pellet altered the rate of antigen release significantly but the level of antibodies produced in mice was only significantly lower for 0.15 mg BSA pellets. Pellets containing BSA were removed from mice and replaced with blank pellets on days 5 and 11; the antibody levels in these mice were significantly lower compared with levels induced by pellets left in situ for 42 days. Pellets caused a localised inflammatory response in a small percentage of mice. It is proposed that cholesterol pellets may provide a practical alternative to multiple injections of vaccine antigens, with particular application in disease prophylaxis in livestock.
Keyword Bovine serum albumin
Cholesterol implant
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 14 Jun 2016, 11:19:00 EST by System User