High-frequency electroporation and maintenance of pUC- and pBR-based cloning vectors in Pseudomonas stutzeri

Pemberton J.M. and Penfold R.J. (1992) High-frequency electroporation and maintenance of pUC- and pBR-based cloning vectors in Pseudomonas stutzeri. Current Microbiology, 25 1: 25-29. doi:10.1007/BF01570078


Author Pemberton J.M.
Penfold R.J.
Title High-frequency electroporation and maintenance of pUC- and pBR-based cloning vectors in Pseudomonas stutzeri
Journal name Current Microbiology   Check publisher's open access policy
ISSN 0343-8651
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1007/BF01570078
Open Access Status Not yet assessed
Volume 25
Issue 1
Start page 25
End page 29
Total pages 5
Publisher Springer-Verlag
Subject 2404 Microbiology
Abstract A number of Escherichia coli cloning vectors, based on ColE1-like replicons, were shown to be maintained in Pseudomonas stutzeri ATCC 17588. A restrictionless mutant of P. stutzeri was isolated, and this strain was used to develop an efficient electroporation system. With the E. coli cloning vector pHSG298, transformation frequencies of up to 2×107 transformants/μg DNA were achieved. This frequency is comparable to that obtained for CaCl2-mediated transformation of E. coli; thus, direct cloning of DNA into P. stutzeri is feasible. As will be discussed, this may prove useful for cloning DNA from high mol% G+C genera in cases in which E. coli is not a suitable heterologous cloning host.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 14 Jun 2016, 11:14:44 EST by System User