Ethanol and protein kinase C in rat brain

Kruger H., Wilce P.A. and Shanley B.C. (1993) Ethanol and protein kinase C in rat brain. Neurochemistry International, 22 6: 575-581. doi:10.1016/0197-0186(93)90032-Z


Author Kruger H.
Wilce P.A.
Shanley B.C.
Title Ethanol and protein kinase C in rat brain
Journal name Neurochemistry International   Check publisher's open access policy
ISSN 0197-0186
Publication date 1993-01-01
Sub-type Article (original research)
DOI 10.1016/0197-0186(93)90032-Z
Volume 22
Issue 6
Start page 575
End page 581
Total pages 7
Subject 1307 Cell Biology
1312 Molecular Biology
2804 Cellular and Molecular Neuroscience
Abstract The effect of chronic ethanol consumption on the catalytic activity of protein kinase C isolated from rat brain was studied in two different ways. Enzyme activity was first measured by phosphorylation of Histone IIIS in vitro. There was no change in the activity of the cytosolic enzyme. Membrane-associated enzyme activity was reduced in the ethanol-treated animal. This difference was not evident if the enzyme was stimulated by arachidonate. The reduction in enzyme activity was confirmed by analysis of the phosphorylation of endogenous substrates in intact synaptosomes. When the binding of the ligand [3H]phorbol dibutyrate was measured by quantitative autoradiography, increased binding to membrane-associated protein kinase C was observed in the CA1 region of the hippocampus but not in other brain regions. These results indicate that ethanol treatment results in a general reduction in membrane-associated protein kinase C activity as measured in vitro but the effect may not be consistent in all brain regions. The differential effect in the CA1 region of the hippocampus may be a reflection of a disruption in the normal regulation of protein kinase C activity in this area and may indicate that this region is a sensitive target for the action of ethanol.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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