Preparation of extracts of culture liquids for gas-chromatographic determination of acidic fermentation products

Doelle H.W. and Manderson G.J. (1969) Preparation of extracts of culture liquids for gas-chromatographic determination of acidic fermentation products. Antonie van Leeuwenhoek, 35 1: 467-478. doi:10.1007/BF02219165


Author Doelle H.W.
Manderson G.J.
Title Preparation of extracts of culture liquids for gas-chromatographic determination of acidic fermentation products
Journal name Antonie van Leeuwenhoek   Check publisher's open access policy
ISSN 0003-6072
Publication date 1969-01-01
Sub-type Article (original research)
DOI 10.1007/BF02219165
Open Access Status Not yet assessed
Volume 35
Issue 1
Start page 467
End page 478
Total pages 12
Publisher Kluwer Academic Publishers
Subject 2404 Microbiology
1312 Molecular Biology
Abstract An extraction method coupled with gas-chromatographic analysis has been devised for the determination of small amounts of C1-C7 branched and straight-chain saturated fatty acids as well as 18 mono-, di- and tricarboxylic acids and related compounds, the latter being analyzed either as their methylesters or as their trimethylsilyl-derivatives. Methods for extracting non-volatile acids or both volatile and non-volatile acids are described for large and small volumes of culture media. The proposed methods have been successfully applied to the determination of non-acidic fermentation products, known amounts of alcohols, and to volatile and non-volatile acids in bacterial fermentation solutions, namely a batch culture of Lactobacillus casei subsp. rhamnosus ATCC 7469. A carbon balance sheet of this organism's metabolic behaviour throughout growth is proposed.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 14 Jun 2016, 10:46:59 EST by System User