Genetic mapping and quantitative trait analysis in the Pinus elliottii Engelm. x Pinus caribaea Mor. hybrid and in Pinus radiata D. don.

Dale, Glenn Thomas (1995). Genetic mapping and quantitative trait analysis in the Pinus elliottii Engelm. x Pinus caribaea Mor. hybrid and in Pinus radiata D. don. PhD Thesis, School of Biological Sciences, University of Queensland. doi:10.14264/uql.2016.411

       
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Author Dale, Glenn Thomas
Thesis Title Genetic mapping and quantitative trait analysis in the Pinus elliottii Engelm. x Pinus caribaea Mor. hybrid and in Pinus radiata D. don.
School, Centre or Institute School of Biological Sciences
Institution University of Queensland
DOI 10.14264/uql.2016.411
Publication date 1995-01-01
Thesis type PhD Thesis
Supervisor Robert Teasdale
Total pages 323
Language eng
Subjects 06 Biological Sciences
Formatted abstract
Genetic improvement of forest tree species is typically constrained by long generation intervals and developmental periods to attain a stable, mature phenotype. Compounded with relatively recent domestication, the most advanced forest tree improvement programs are now only in the first three or four generations of selection. Genetic mapping provides a framework of reference points throughout the genome enabling systematic detection and characterization of individual Mendelian loci contributing to the control of quantitative traits of interest. Practical application of this information through genetic marker assisted selection offers significant potential to advance the rate of forest tree improvement. However, the application of this technology to forest tree species also poses unique problems not encountered in relatively short generation agricultural species. 

Rapid advances in both genetic marker technology and analytical techniques for genome analysis in forest tree species necessitated a review of these subjects. Random amplified polymorphic DNA markers were identified for application and evaluation in the present study, while identification and characterization of QTL by both interval mapping and conventional ANOVA each provided particular advantages. Even still, the rapid rate of advancement in both these areas warrants continual review of these subjects. 

By exploiting the biology of the haploid megagametophyte, dominant RAPD markers were employed in constructing reference genetic maps for an individual Pinus elliottii x Pinus caribaea F1 hybrid tree, and for an individual Pinus radiata tree. Efficient application of RAPD markers was demonstrated through appropriate pre-screening to identify suitably informative markers. Each genetic map was used to genotype full-sib F2 progeny of the former individual, and half-sib progeny of the latter. The transfer of RAPD marker information between generations was shown to be reliable provided rigorous attention is paid to experimental detail, and marker segregation patterns are conservatively scored.   ..........................................
Keyword Plant genome mapping
Pine -- Genetics
Forest genetics
Additional Notes Spine title: Genetic mapping and quantitative trait analysis in Pinus species.

Document type: Thesis
Collection: UQ Theses (RHD) - UQ staff and students only
 
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