A new Orbivirus isolated from mosquitoes in north-western Australia shows antigenic and genetic similarity to Corriparta virus but does not replicate in vertebrate cells

Harrison, Jessica J., Warrilow, David, McLean, Breeanna J., Watterson, Daniel, O'Brien, Caitlin A., Colmant, Agathe M.G., Johansen, Cheryl A., Barnard, Ross T., Hall-Mendelin, Sonja, Davis, Steven S., Hall, Roy A. and Hobson-Peters, Jody (2016) A new Orbivirus isolated from mosquitoes in north-western Australia shows antigenic and genetic similarity to Corriparta virus but does not replicate in vertebrate cells. Viruses, 8 5: e141.1-e141.15. doi:10.3390/v8050141


Author Harrison, Jessica J.
Warrilow, David
McLean, Breeanna J.
Watterson, Daniel
O'Brien, Caitlin A.
Colmant, Agathe M.G.
Johansen, Cheryl A.
Barnard, Ross T.
Hall-Mendelin, Sonja
Davis, Steven S.
Hall, Roy A.
Hobson-Peters, Jody
Title A new Orbivirus isolated from mosquitoes in north-western Australia shows antigenic and genetic similarity to Corriparta virus but does not replicate in vertebrate cells
Journal name Viruses   Check publisher's open access policy
ISSN 1999-4915
Publication date 2016-05-20
Sub-type Article (original research)
DOI 10.3390/v8050141
Open Access Status DOI
Volume 8
Issue 5
Start page e141.1
End page e141.15
Total pages 15
Place of publication Basel, Switzerland
Publisher M D P I AG
Language eng
Formatted abstract
The discovery and characterisation of new mosquito-borne viruses provides valuable information on the biodiversity of vector-borne viruses and important insights into their evolution. In this study, a broad-spectrum virus screening system, based on the detection of long double-stranded RNA in inoculated cell cultures, was used to investigate the presence of novel viruses in mosquito populations of northern Australia. We detected and isolated a new virus (tentatively named Parry’s Lagoon virus, PLV) from Culex annulirostris, Culex pullus, Mansonia uniformis and Aedes normanensis mosquitoes that shares genomic sequence similarities to Corriparta virus (CORV), a member of the Orbivirus genus of the family Reoviridae. Despite moderate to high (72.2% to 92.2%) amino acid identity across all proteins when compared to CORV, and demonstration of antigenic relatedness, PLV did not replicate in several vertebrate cell lines that were permissive to CORV. This striking phenotypic difference suggests that PLV has evolved to have a very restricted host range, indicative of a mosquito-only life cycle.
Keyword Culex annulirostris
Arbovirus
Double-stranded RNA
Insect-specific virus
Mosquito
Orbivirus
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 5 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 27 May 2016, 19:54:04 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences