A two-stage method to correct aberrations induced by slide slant in bright-field microscopy

Fan, Yilun and Bradley, Andrew P. (2016) A two-stage method to correct aberrations induced by slide slant in bright-field microscopy. Micron, 87 18-32. doi:10.1016/j.micron.2016.05.001

Author Fan, Yilun
Bradley, Andrew P.
Title A two-stage method to correct aberrations induced by slide slant in bright-field microscopy
Journal name Micron   Check publisher's open access policy
ISSN 0968-4328
Publication date 2016-08-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.micron.2016.05.001
Open Access Status Not Open Access
Volume 87
Start page 18
End page 32
Total pages 15
Place of publication Kidlington, Oxford United Kingdom
Publisher Pergamon Press
Language eng
Subject 1315 Structural Biology
1307 Cell Biology
Abstract To achieve optimal image quality in bright field microscopy, the slide surface should be perpendicular to the optical axis of the microscope. However, in the recently proposed “slanted scan” slide acquisition technique, scan speed is increased by purposely slanting the slide by a small angle (of 3–5°) so that multiple focal depths can be imaged simultaneously. In this case, the slanted slide introduces a bend in the point spread function (PSF), resulting in a coma and other aberrations that degrade image quality. In this paper, we propose a two-stage deconvolution method specifically designed to correct the aberrations induced by a slanted scan, but with general applicability to high-resolution bright-field microscopy. Specifically, we initially apply phase deconvolution to correct the dominating coma aberration, before applying a conventional semi-blind deconvolution method to further improve image resolution and contrast. We also propose a novel method to estimate the degree of coma aberration and the PSF of the optics utilising actual cytology specimens. The efficacy of the proposed algorithm is demonstrated quantitatively on simulated data, against a ground-truth (object) image, and qualitatively on cervical cytology specimens. Results demonstrate both improved convergence speed of the two-stage approach, especially when correcting the bend in the PSF, and a resultant image quality that is comparable to a conventionally (flat) scanned specimen.
Keyword Virtual microscopy
Tilted plane parallel plate
Coma aberration
Phase deconvolution
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID FT110100623
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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School of Information Technology and Electrical Engineering Publications
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