The in vitro and in vivo antiviral properties of combined monoterpene alcohols against West Nile virus infection

Pliego Zamora, Adriana, Edmonds, Judith H., Reynolds, Maxwell J., Khromykh, Alexander A. and Ralph, Stephen J. (2016) The in vitro and in vivo antiviral properties of combined monoterpene alcohols against West Nile virus infection. Virology, 495 18-32. doi:10.1016/j.virol.2016.04.021


Author Pliego Zamora, Adriana
Edmonds, Judith H.
Reynolds, Maxwell J.
Khromykh, Alexander A.
Ralph, Stephen J.
Title The in vitro and in vivo antiviral properties of combined monoterpene alcohols against West Nile virus infection
Formatted title
The in vitro and in vivo antiviral properties of combined monoterpene alcohols against West Nile virus infection
Journal name Virology   Check publisher's open access policy
ISSN 1096-0341
0042-6822
1089-862X
Publication date 2016-08-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.virol.2016.04.021
Open Access Status Not Open Access
Volume 495
Start page 18
End page 32
Total pages 15
Place of publication Waltham, MA United States
Publisher Academic Press
Language eng
Formatted abstract
West Nile Virus (WNV) is a mosquito-borne flavivirus that can cause neuroinvasive disease in humans and animals for which no therapies are currently available. We studied an established combination of monoterpene alcohols (CMA) derived from Melaleuca alternifolia, against WNV infection. The in vitro results show that CMA exhibits virucidal activity, as well as reduces the viral titres and percentage of infected cells. The antiviral mechanism of action of CMA was studied. We found that CMA did not alter the intracellular pH, neither induced apoptosis, but did induce cell cycle arrest in the G0/G1-phase although that was not the antiviral mechanism. Furthermore, we tested CMA in vivo using IRF 3−/−/7−/−mice and it was found that CMA treatment significantly delayed morbidity due to WNV infection, reduced the loss of body weight and reduced the viral titres in brain. These findings suggest that CMA could be a therapeutic agent against WNV infection.
Keyword Flavivirus
Virucidal
Antiviral agent
Cell cycle
Mice
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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