Differences in muscle transcriptome among pigs phenotypically extreme for fatty acid composition

Puig-Oliveras, Anna, Ramayo-Caldas, Yuliaxis, Corominas, Jordi, Estelle, Jordi, Perez-Montarelo, Dafne, Hudson, Nicholas J., Casellas, Joaquim, Folch, Josep M. and Ballester, Maria (2014) Differences in muscle transcriptome among pigs phenotypically extreme for fatty acid composition. PLoS ONE, 9 6: . doi:10.1371/journal.pone.0099720

Author Puig-Oliveras, Anna
Ramayo-Caldas, Yuliaxis
Corominas, Jordi
Estelle, Jordi
Perez-Montarelo, Dafne
Hudson, Nicholas J.
Casellas, Joaquim
Folch, Josep M.
Ballester, Maria
Title Differences in muscle transcriptome among pigs phenotypically extreme for fatty acid composition
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-06-13
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0099720
Open Access Status DOI
Volume 9
Issue 6
Total pages 11
Place of publication San Francisco, United States
Publisher Public Library of Science
Language eng
Formatted abstract
Background: Besides having an impact on human health, the porcine muscle fatty acid profile determines meat quality and taste. The RNA-Seq technologies allowed us to explore the pig muscle transcriptome with an unprecedented detail. The aim of this study was to identify differentially-expressed genes between two groups of 6 sows belonging to an Iberian x Landrace backcross with extreme phenotypes according to FA profile.

Results: We sequenced the muscle transcriptome acquiring 787.5 M of 75 bp paired-end reads. About 85.1% of reads were mapped to the reference genome. Of the total reads, 79.1% were located in exons, 6.0% in introns and 14.9% in intergenic regions, indicating expressed regions not annotated in the reference genome. We identified a 34.5% of the intergenic regions as interspersed repetitive regions. We predicted a total of 2,372 putative proteins. Pathway analysis with 131 differentially-expressed genes revealed that the most statistically-significant metabolic pathways were related with lipid metabolism. Moreover, 18 of the differentially-expressed genes were located in genomic regions associated with IMF composition in an independent GWAS study in the same genetic background. Thus, our results indicate that the lipid metabolism of FAs is differently modulated when the FA composition in muscle differs. For instance, a high content of PUFA may reduce FA and glucose uptake resulting in an inhibition of the lipogenesis. These results are consistent with previous studies of our group analysing the liver and the adipose tissue transcriptomes providing a view of each of the main organs involved in lipid metabolism.

Conclusions: The results obtained in the muscle transcriptome analysis increase the knowledge of the gene regulation of IMF deposition, FA profile and meat quality, in terms of taste and nutritional value. Besides, our results may be important in terms of human health.
Keyword Multidisciplinary Sciences
Science & Technology - Other Topics
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID AGL2011-29821-C02
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
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Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 19 times in Scopus Article | Citations
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